精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11233™THLE-3 細(xì)胞,人肝永生化細(xì)胞

THLE-3 細(xì)胞,人肝永生化細(xì)胞

簡要描述:THLE-3 細(xì)胞,人肝永生化細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-11233™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問  量:1774

產(chǎn)品分類

Product Category

詳細(xì)介紹

THLE-3 細(xì)胞,人肝永生化細(xì)胞

traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 0.1% Soybean Trypsin inhibitor  and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

6.Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new coated culture vessels.

7.Place culture vessels in incubators at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

THLE-3 細(xì)胞,人肝永生化細(xì)胞

Flask Coating

1.Prepare a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin (BSA) dissolved in culture medium. Store pre-prepared Coating Solution at 4°C in cold room for up to 3 months.

2.For a growth area of 75 cm2, add 4.5 mL of the fibronectin/collagen/BSA solution and rock gently to coat the entire surface.

3.Incubate the freshly coated vessel(s) in a 37°C incubator overnight (it is preferable to use tissue culture vessels with tightened, plug-seal caps to prevent evaporation during the coating process).

4.Store coated flasks with solution at room temperature, light protected, up to 1 month. Suction off solution before plating cells.


Cryopreservation  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11,12

D13S317: 13

D16S539: 11,12

D5S818: 13

D7S820: 8,10

THO1: 8,9.3

TPOX: 6,9

vWA: 17,18

Name of Depositor  National Cancer Institute

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
老师好大好爽我要喷水了视频 | 中文字幕亚洲乱码熟女在线萌芽| 国产精品无码久久久久不卡| 思思久久精品在热线热| 无码天堂亚洲国产AV久久| 爱上黑道女友| 精品人妻久久久久久888| 久久久久久久人妻无码中文字幕爆| 偷窥50个美女撒尿高清| 色狠狠一区二区三区香蕉| 又黄又爽又色的视频| 国产麻豆剧传媒精品国产av| 日产一二三区别免费必看| 办公室里呻吟的丰满老师电影| 免费av网站| 肥白大屁股bbwbbwhd| 日韩视频在线观看| 粗大的内捧猛烈进出a片| 一天不日b浑身难受| 最好朋友的老公上我了怎么办| 曰曰摸日日碰夜夜爽歪歪| 奇米777 米奇影视狠狠| 人妻女友娇妻沉沦系列| 午夜精品久久久久久毛片| 国产精品久久久久久无码| 亚洲欧美人成无码苍井空| 隔壁人妻偷人bd中字| 小娟翁止熄痒禁伦短文合集俏佳人 | 亚洲色熟女图激情另类图区| 国产成人精品免高潮在线观看| 征服同学人妇系列陈露露3 | 国产A级毛片久久久久久精品| 久久精品国产精品亚洲毛片 | 国产成人精品久久| www夜插内射视频网站| 车上他吃我奶进我下面| 乳罩脱了喂男人吃奶视频| 婷婷五月色丁香综缴情| 99精品久久毛片a片| 最近免费中文字幕mv免费高清版| 夜躁狠狠综合亚洲色噜噜狠狠|