精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11233™THLE-3 細(xì)胞,人肝永生化細(xì)胞

THLE-3 細(xì)胞,人肝永生化細(xì)胞

簡要描述:THLE-3 細(xì)胞,人肝永生化細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-11233™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問  量:1774

產(chǎn)品分類

Product Category

詳細(xì)介紹

THLE-3 細(xì)胞,人肝永生化細(xì)胞

traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 0.1% Soybean Trypsin inhibitor  and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

6.Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new coated culture vessels.

7.Place culture vessels in incubators at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

THLE-3 細(xì)胞,人肝永生化細(xì)胞

Flask Coating

1.Prepare a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin (BSA) dissolved in culture medium. Store pre-prepared Coating Solution at 4°C in cold room for up to 3 months.

2.For a growth area of 75 cm2, add 4.5 mL of the fibronectin/collagen/BSA solution and rock gently to coat the entire surface.

3.Incubate the freshly coated vessel(s) in a 37°C incubator overnight (it is preferable to use tissue culture vessels with tightened, plug-seal caps to prevent evaporation during the coating process).

4.Store coated flasks with solution at room temperature, light protected, up to 1 month. Suction off solution before plating cells.


Cryopreservation  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11,12

D13S317: 13

D16S539: 11,12

D5S818: 13

D7S820: 8,10

THO1: 8,9.3

TPOX: 6,9

vWA: 17,18

Name of Depositor  National Cancer Institute

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
chinese山西老熟女bbw| 乔家的儿女电视剧全集免费播放| 欧美一区二区三区成人片在线| 欧美乱妇狂野欧美在线视频| jizz国产精品| 女女互磨互喷水高潮les呻吟| 老师露出两个奶球让我吃奶头| 在熟睡夫面前侵犯我在线播放| 强壮公弄得我次次高潮小说| 摄像头东北对白清晰| 国产亲妺妺乱的性视频| 妓院里的中国姑娘| 啊灬啊灬啊灬快灬高潮了听书| 真人高清实拍女处被破的视频| 人妻少妇被猛烈进入中文字幕| 婷婷丁香五月久久综合啪啪图区| 精品久久久久久无码人妻黑人 | 高h各种姿势调教1v1| 99久久久精品免费观看国产| 老板含着她的花蒂啃咬高潮的视频 | 国产老头老太作爱视频| 精品无码人妻一区二区三区| 无码天堂亚洲国产AV久久| 清区二三区国产好的精华液| 人人妻人人玩人人澡人人爽| 娇妻玩4P被三个男人伺候电影 | 吴北唐紫怡小说免费阅读| 成人av视频免费观看| 伊人情人综合网| 日本a级作爱片金瓶双艳| 免费毛片在线看不用播放器| 粗大挺进亲女h晓晓| av视频在线观看| 将军边走边挺进她的小泬| 故意短裙公交车被强好爽h| 高潮aaa人人爽人人爱| 成人免费无遮挡无码黄漫视频| 2023国产麻豆剧传媒鱿鱼游戏| 99re在线精品国产欧美| 做床爱30分钟免费观看| 真人试看体验区120秒|