精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2922EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

簡要描述:EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2922
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問  量:1987

產(chǎn)品分類

Product Category

詳細(xì)介紹

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

ATCC® Number:CRL-2922™    Price:
Designations:EA.hy926

Depositors:CS Edgell

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


Source:Tissue: somatic cell hybrid

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may gbe required for the transfer of this ATCC material. Anyone purchasin ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

Restrictions:For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression:Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR):Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17



Comments:The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C


Subculturing:Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.
    Interval: Twice a week
    Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended
    Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete Growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402



References:92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
精品人妻av一区二区三区| 免费看裸体美女脱了衣服露视频胸 | 久久久综合精品一区二区三区 | 成品人视频推荐素材网站| 亚洲av无码一区二区三区| 无码一区二区三区| 欧美另类69xxxxx| 美国式禁忌在线观看| 寂寞少妇扒开双腿猛烈进入免费看| 99re8在这里只有精品2| 美女100%裸体无内衣内裤| 护士猛少妇色xxxxx猛叫| 国产精品视频一区二区三区不卡| 蜜桃狠狠色伊人亚洲综合网站| 寡妇大j8又粗又大| 亚洲 欧洲 日产| 久久精品国产| chinese学生高中男gay| 野花日本hd免费高清版7| 一区二区三区毛a片特级| av电影网站| 免费国产又色又爽又黄的网站| 岳的大肥坹视频hd| 久久久久久久人妻无码中文字幕爆| 性一交一乱一乱一视频| 老熟女重囗味hdxx70星空| 国产在线av一区二区麻豆董小宛 | 女警乳链乳环蒂环铃铛调教| 57歳の熟女セックス| chinese山西老熟女bbw| 无码精品黑人一区二区三区| 精品人伦一区二区三区蜜桃| 国产中文精品无码欧美综合小说| 女性成人用品| 我和公发生了性关系视频| 日韩亚洲变态另类中文| 10后学生小嫩嫩xxx| 最新中文字幕av专区| 公主恋人ova| 性裸交a片a∨天传媒公司| 国产福利视频在线观看|