精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CCL-186IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

簡要描述:IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CCL-186
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2000

產(chǎn)品分類

Product Category

詳細(xì)介紹

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

ATCC® Number:  CCL-186™

Designations:  IMR-90

Depositors:   WW Nichols

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes:  G6PD, B

Age:  16 weeks gestation

Gender:  female

Ethnicity:  Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
无码a片| 超碰caoporen97人人| 人妻精品久久久久中文字幕69| 国模冰莲自慰肥美胞极品人体图| 高潮搐痉挛潮喷av| 国产在线拍揄自揄视精品| 班长用她的玉足白丝帮我爽| 奶头挺立呻吟高潮av全片| 交换年轻夫妇HD中文字幕3D| 美女100%裸体| 东北浓毛老妇国语对白| 亚洲av无码久久| 国产99精品视频一区二区三区| 国模少妇一区二区三区咪咕| 无码精品人妻一区二区三区漫画| 东北熟女bbwbbw喷水| 国产人妻大战黑人20p| 粗大浓稠硕大噗嗤噗嗤H| 女人被狂躁免费看30分钟| 色欲av色臀av精东传媒| 无码免费一区二区三区| 少妇人妻人伦a片| 图书馆H含着粉嫩小奶头H漫画| 男友把舌头都伸进我的嘴巴里了 | 国产又色又爽又高潮免费| 放荡亲女养成h| 公交车上拨开少妇内裤进入| 精品人妻无码一区二区三区不卡| 青柠在线影院观看日本| 99精品人妻无码专区在线视频区| 公与淑婷厨房猛烈进出| 中文字幕人妻丝袜乱一区三区| 国产又黄又爽又色视频免费软件| 99久久久精品免费观看国产| 含着她的花蒂啃咬高潮| 亚洲国产精华液2020| 天干天干天啪啪夜爽爽99| 十大乱翁系列小说| 亚洲av无码专区在线观看下载| 妓女精品国产噜噜亚洲av| 娇妻借朋友高h繁交h|