精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2234SNU-449細(xì)胞, 人肝癌細(xì)胞

SNU-449細(xì)胞, 人肝癌細(xì)胞

簡要描述:SNU-449細(xì)胞, 人肝癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,提供 *培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2234
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1929

產(chǎn)品分類

Product Category

詳細(xì)介紹

SNU-449細(xì)胞, 人肝癌細(xì)胞(ATCC® CRL-2234)

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissue

liver

Product Formatfrozen
Morphologyepithelial; diffusely spreading cells
Culture Propertiesadherent
Biosafety Level2  [Cells contain Hepatitis B virus]
Diseasegrade II-III/IV,hepatocellular carcinoma
Age52 years
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
Karyotypeaneuploid; modal number = 57
Derivation

SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Clinical Data

52 years

Asian

male

SNU-449細(xì)胞, 人肝癌細(xì)胞

Comments

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.

Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single or double nucleus.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
SubculturingProtocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久艾草久久综合精品无码国产| 少妇又紧又色又爽又刺激视频 | 国产精品人人做人人爽人人添 | 全黄裸片一29分钟免费真人版| 99久久精品免费看国产| 大陆农村妇女老bbwbbw| 西西gogo顶级艺术人像摄影 | 荡公乱妇11部分| 日韩在线视频| 人妻夜夜爽天天爽三区麻豆av| 男女无遮挡猛进猛出免费视频| 宝贝腿开大点我添添公口述 | 人妻女友娇妻沉沦系列| 银行娇妻1一14全文阅读| 免费看av| 国产精品久久午夜夜伦鲁鲁| japanese日本丰满少妇| 色老太bbwbbwbbw高潮| 我美艳丰满的吗咪| 国产娇小粉嫩学生免费网站| 玩50岁四川熟人A片| 亚洲日韩乱码一区二区三区四区| 娜娜的yin荡生涯h全文| 小少妇bbbbbbbbbbbb| 用舌头去添女人下面是不是真爱 | 国模精品一区二区三区| 500篇艳妇短篇合午夜人屠| 中文字幕在线播放| 国产伦精品一区二区三区免费| 成人用品进货批发网| 喘声2分30秒戴好耳机| 办公室撕开奶罩揉吮奶漫画| 男男车车的车车网站W98免费| 寝室里的高潮(h)| 午夜福利电影| 美女露?0的奶头无挡挡| 男生自慰出精gay2022| 中学生广播体操舞动青春| 日韩人妻无码一区二区三区免费| 中字幕视频在线永久在线观看免费| 成免费crm在线看系统|