精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>FL62891人肝細(xì)胞

人肝細(xì)胞

簡(jiǎn)要描述:CRL-11005 FL62891 人肝細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):FL62891
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1928

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-11005 FL62891 人肝細(xì)胞

Designations: FL 62891
Depositors: ImClone Systems Inc.
Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen


Medium & Serum: San)ee PropagationATCC® Number: CRL-11005™ Price: $353.00


Growth Properties: adherent

Organism: Homo sapiens (hum

Morphology: fibroblast

CRL-11005 FL62891 人肝細(xì)胞

Source: Organ: liver

Cell Type: immortalized with SV40 large T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Age: fetus

Comments: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 32.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 32°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993

70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994

70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996

70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997

70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998

70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999



CRL-11005 FL62891



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
色噜噜久久综合伊人超碰| 艳妇荡女欲乱A片在线观看| 一层一层剥掉女生的衣服的游戏| 高清破外女出血视频全过程| 自w到高c的25种方法带图| 亚洲av天堂一区二区香蕉| 无码毛片aaa在线| 久久中文字幕人妻熟av女蜜柚m | 全是肉肉的说说| 掀起衣服含着奶头h| 五十路息与子在线播放藤崎樱| 久爱99爱九九av视频在线| 日本丰满大乳人妻无码苍井空| 欧美老熟妇xb水多毛多| 国产老妇伦国产熟女老妇视频| 隔壁人妻偷人bd中字| 奶水h人妻销魂共妻高h| 要灬要灬再深点受不了好舒服| 免费追剧APP软件| 日本va欧美va精品发布| 三攻一受4p肉调教| 掀起衣服含着奶头h| 蜜臀AV精品一区二区三区| 日韩性做爰免费a片aa片| 一本久道久久综合狠狠爱| 极品新婚夜少妇真紧| 男受被做哭激烈娇喘gv视频| 国产午夜精品一区理论片飘花 | 老农民里老干棒媳妇回来了吗| 丰满岳乱妇在线观看中字无码| 亚洲精品粉嫩小泬18p| 小舞被肉干高h潮文不断| 蜜臀AV夜夜澡人人爽人人| 国产在线观看国偷精品产拍| 久久久久亚洲av片无码下载蜜桃 | 门卫老李干了校花琦琦| 一区二区三区| 国产三级农村妇女系列| 捏胸吻胸添奶头gif动态图| 日韩久久无码免费毛片软件| 草莓视频app黄|