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FDC-P1 小鼠正常骨髓細(xì)胞

簡(jiǎn)要描述:CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-12103
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2161

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CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞的詳細(xì)介紹

CRL-12103 FDC-P1 小

鼠正常骨髓細(xì)胞

ATCC® Number:CRL-12103™  Price:$355.00
Designations:FDC-P1

Depositors:University of Virginia Patent Foundation

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

G
th Properties:suspension
Organism:Mus musculus (mouse)
Morphology:lymphoblas
row




t


Source:Organ: bone marrow
Strain: DBA/2
Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-12103

Applications:transfection host (Nucleofection technology from Lonza)

Antigen Expression:Thy-1.2 (24%)

Cytogenetic Analysis:diploid

Comments:The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).

They are dependent upton WEHI-3 conditioned medium (WEHI-3CM) for continued growth.



Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040).
Temperature: 37.0°C


Subculturing:Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature


















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