精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>小鼠腫瘤細胞、癌細胞>CRL-6475B16F10 小鼠黑色素瘤高轉(zhuǎn)移細胞

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細胞

簡要描述:CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-6475
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2039

產(chǎn)品分類

Product Category

詳細介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細胞

ATCC® Number:CRL-6475™  Price:$269.00
Designations:B16-F10

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:melanocyte
B16F10 小鼠黑色素瘤高轉(zhuǎn)移細胞


Source:Organ: skin
Strain: C57BL/6J
Disease: melanoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-6475

Applications:transfection host (technology from amaxa)

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase



CRL-6475
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
野外吮她的花蒂两男一女| 大秦赋全集1-78集免费观看| 天天做天天爱夜夜爽| 成人视频在线观看| 野外做受又硬又粗又大视频√| 人妻巨大乳一二三区| 国产欧美一区二区三区| 日韩无码av| 房东天天吃我奶躁我| 白嫩人妻娇喘欲仙欲死| 免费观看又色又爽又黄的| 500篇艳妇短篇合午夜人屠| 大又大又粗又硬又爽少妇毛片| 国产精品沙发午睡系列| 宝贝∽好硬∽好爽一再...| 老师揉捏爆乳巨胸挤奶视频| 不许穿内裤随时挨C调教H| 国产成人精品三上悠亚久久| 销魂美女图库| 国产成人无码aa精品一区| 高潮搐痉挛潮喷av| 男女做爰猛烈吃奶摸动态图| 日本人是sb吗| 被猛男cao烂的小男生gv| 日韩毛片人妻久久蜜桃传媒| 成全视频在线观看免费高清版| 再深点灬舒服灬太大了添a片小说| 女人第一次一进去就出血| 东京爱情动作故事| 五十路熟女人妻一区二区 | 曰本丰满熟妇xxxx性| 被黑人伦流澡到高潮HNP动漫 | 免费a级毛片| 他一边曰一边吃我奶小说免看 | 亚洲va在线va天堂va无码| 亚洲妇女无套内射精| 欧美色综合天天久久综合精品| 精品人伦一区二区三区蜜桃| 黑人猛挺进小莹的体内视频| 狠狠综合久久av一区二区三区| 国产精品久久久久久久9999|