精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

簡(jiǎn)要描述:CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2569

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

ATCC® Number:CRL-2007™Price:$429.00
Designations:JB6 Cl 30-7b

Depositors:NH Colburn

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: skin
Strain: BALB/c
Tissue: epidermis
Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Tumorigenic:CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

Age:newborn

Comments:This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays (see also ATCC CRL-2002 - RT101, ATCC CRL-2010 - JB6 Cl 41-5a and ATCC CRL-2012 - T36274).

The JB6 Cl 30-7b cell line was derived from primary cultures of neonatal BALB/c epidermal cells that had been treated with dimethylsulfoxide (DMSO, 0.01 to 0.1 %).

The line is resistant to promotion of transformation (P-) by phorbol esters.

These cells can be used in comparisons with P+ cells for studying differential responses to tumor promoters.

Never allow the cells to become confluent.

The cells must be carried at low density to avoid spontaneous transformation.



Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Temperature: 37.0°C


Subculturing:Protocol: The cells must be carried at low density to avoid spontaneous transformation.Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculation density of 2 x 104 viable cells per 25 sq. cm. flask is recommended or a spilt ratio of 1:6 to 1:8.6. Incubate cultures at 37°C.
Subc*tion Ratio: An inoculation density of 2 X 10 exp4 viable cells per 25 sq. cm. flask is recommended
Medium Renewal: 1 to 2 times per week

Rap the flask smartly against a hard surface to get a single cell suspension.

Add fresh medium, aspirate and dispense into new flasks. Culture at 36C in a humidified atmosphere of 5% CO2 - 95% air.



References:22309: Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803

22427: Colburn NH, et al. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967

22908: Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322

22959: Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502

23279: Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
息与子猛烈交尾在线播放| 老男人把舌头伸进我下面| 大战丰满人妻性色av偷偷红豆| 美女网站色| 少妇与大狼拘作爱性a片| 男人最想听到女人叫床词| 性久久久| 免费观看片的app下载| 国产最猛性xxxx69交| 两根巨物一起三p白洁| china中国bingo视频| 高潮vpswindows国产乱| 女人与公驹交酡全过程| 亚洲av无码一区东京热久久| 性xxxxfreexxxxxvideo| free欧美性xxxxhd| 欧美日韩在线视频| 成人免费视频在线观看| 精品无码欧美一区二区三区不卡| b站禁止转播404入口| 国产真实乱了露脸在线观看| 扒开双腿疯狂进出爽爽爽动态照片| 啊灬啊灬啊灬啊灬高潮了| 欧美高清videossexo| 午夜欧美精品久久久久久久| 富婆一对一刺激交友| 新婚妻子1-3部20章| 羞耻play公共场所h调教| 国产伦精品一区二区三区免费| 学生16女人毛片免费视频| 凸凹人妻人人澡人人添| 国内精品久久久久影院欧美| 国产伦精品一区二区三区妓女| 国产超碰人人模人人爽人人添| 国产特级毛片a片www| 欧美zc0o人与善交另类a片| 亚洲熟女少妇一区二区三区| 女人和拘做受全过程免费| 甜蜜惩罚我是看守专用宠物| 国产边打电话边被躁视频| 日本护士毛茸茸|