精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1690 T98G 人多形性惡性膠質(zhì)瘤細(xì)胞

CRL-1690 T98G 人多形性惡性膠質(zhì)瘤細(xì)胞

簡要描述:CRL-1690 T98G 人多形性惡性膠質(zhì)瘤細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2715

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1690 T98G  人多形性惡性膠質(zhì)瘤細(xì)胞

ATCC® Number:CRL-1690™Price:$279.00
Designations:T98G [T98-G]

Depositors:GH Stein

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast


Source:Organ: brain

Disease: glioblastoma multiforme



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host

Tumorigenic:CRL-1690 T98G  人多形性惡性膠質(zhì)瘤細(xì)胞

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13

D16S539: 13

D5S818: 10,12

D7S820: 9,10

THO1: 7,9.3

TPOX: 8

vWA: 17,20



Cytogenetic Analysis:This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy.

Age:61 years  CRL-1690 T98G  人多形性惡性膠質(zhì)瘤細(xì)胞

Gender:male

Ethnicity:Caucasian

Comments:When deprived of serum or when crowded, the cells enter a viable G1 arrested state.

The cells are anchorage independent.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

CRL-1690 T98G  人多形性惡性膠質(zhì)瘤細(xì)胞

  • Remove and discard culture medium.

  • Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  • Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

  • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

  • Incubate cultures at 37C.

CRL-1690 T98G  人多形性惡性膠質(zhì)瘤細(xì)胞

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:22322: Stein GH. T98G: an anchorage-independent human tumor cell line that exhibits stationary phase G1 arrest in vitro. J. Cell. Physiol. 99: 43-54, 1979. PubMed: 222778

23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221

32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024


CRL-1690 T98G  人多形性惡性膠質(zhì)瘤細(xì)胞




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
在线观看免费视频| 福利cosplayh裸体の福利| 巨茎人妖自慰出精| 少妇撒尿W搡BBB搡WBBB搡| 阿娇张开两腿实干13分钟图片| 免费观看性欧美大片无片| 欧美av蜜桃一区二区蜜桃| 婷婷亚洲五月色综合久久| 亚洲av成人片色在线观看高潮| 亚洲视频在线观看| 欧美性人人天天夜夜摸| 无码熟妇av人妻又粗又大| 我把小嫩批日出水的视频| 最近免费中文字幕中文高清百度| 德国free性video极品| 最穷的山村妇女做爰| 国产精品一区二区av| 欧美 变态 另类 人妖| 人妻(高H)| 母亲とが话しています免费| 国语自产偷拍精品视频偷拍图片| 在线观看视频| 欧美a级suv大全| 亚洲精品人成影精品院| 亚洲电影在线观看| 亚洲国产av精品一区二区蜜芽| 最近国语视频在线观看免费播放 | 把她日出水了好紧大爽了视频| 国产69精品麻豆久久久久| 6080yyy午夜理论aa片| 国产乱妇无乱码大黄aa片| 少妇特殊按摩高潮惨叫无码| 无码人妻丰满熟妇啪啪欧美| porno日本| 国产综合在线观看| 国产农妇精品xxx| 偷偷藏不住小说免费阅读| 亚洲精品又粗又大又爽a片| 成人精品视频99在线观看免费| 国产一区二区草草影院| 男男情趣玩具play高h|